primary antibodies against p53  (Affinity Biosciences)

Journal: bioRxiv

Article Title: Functional inactivation of the telomerase chaperone TCAB1 primes cells for the activation of ALT in osteosarcoma

doi: 10.64898/2026.01.02.691864

Figure Lengend Snippet: A) Schematic representation of the orientation of the TP53 and WRAP53/TCAB1 genes within the genome. B) Representative image of an agarose gel from a TRAP assay performed on OS tumors. SJSA1 serves as a telomerase positive control and buffer alone serves as the negative control. IC; internal PCR control, Telomerase positive; +, Telomerase negative; -. C) Representative TEL-FISH result for SJSA1(negative control) and indicated OS tumors. Arrows indicate cells with ultra bright telomeric foci. D) Representative TP53-IHC result for SJSA1 (expresses mutant TP53) and OS tumors with complete loss of TP53 expression. E) TCAB1-IHC result for SJSA1(positive control) and indicated OS tumors with loss of TCAB1 expression.

Article Snippet: For p53, slides were steamed in pH6.0 citrate-based unmasking solution (Cat# H-3300, Vector Laboratories) for 30 min, blocked with ReadyProbes Endogenous HRP and AP Blocking Solution (Invitrogen, #R37629) and Protein Block (#ab64226, abcam), then were incubated overnight at 4C in mouse monoclonal primary antibody against p53 (1:50 dilution, Cat# sc-126, Santa Cruz Biotechnology).

Techniques: Agarose Gel Electrophoresis, TRAP Assay, Positive Control, Negative Control, Control, Mutagenesis, Expressing

Journal: bioRxiv

Article Title: Functional inactivation of the telomerase chaperone TCAB1 primes cells for the activation of ALT in osteosarcoma

doi: 10.64898/2026.01.02.691864

Figure Lengend Snippet: A) Categorical heatmap summarizes the SVs for TP53, TCAB1, and ALT associated genes that were identified for each of the five OS tumors analyzed by WGS. Complex (COM) and translocations (TRA) are represented as dark blue and light blue respectively. B) Illustration of the specific SVs identified in each of the five OS tumors analyzed by WGS. C) PCR analysis conducted on the 3.6 KB flanking region between TP53 and TCAB1 that identifies deletion of this locus in tumors 0560-000 and CPRIT 1981. Red lines represent location of primers D) Validation of the translocation event between JARID2-TCAB1 on Ch6:17 in the tumor OS39. HEK293 serves as a negative control for T6:17.

Article Snippet: For p53, slides were steamed in pH6.0 citrate-based unmasking solution (Cat# H-3300, Vector Laboratories) for 30 min, blocked with ReadyProbes Endogenous HRP and AP Blocking Solution (Invitrogen, #R37629) and Protein Block (#ab64226, abcam), then were incubated overnight at 4C in mouse monoclonal primary antibody against p53 (1:50 dilution, Cat# sc-126, Santa Cruz Biotechnology).

Techniques: Biomarker Discovery, Translocation Assay, Negative Control

Journal: bioRxiv

Article Title: Functional inactivation of the telomerase chaperone TCAB1 primes cells for the activation of ALT in osteosarcoma

doi: 10.64898/2026.01.02.691864

Figure Lengend Snippet: A) Categorical heatmap summarizes ALT status, SVs for TP53, TCAB1, and ALT associated genes that were identified for all OS tumors analyzed by WGS. B) Relative percentage of ALT- and ALT+ samples with a SV affecting the TP53 and TCAB1 genomic regions are represented as a stacked bar chart where deletions, inversions, translocations and complex events are orange, yellow, light blue and dark blue respectively. C) Categorical heatmap summarizes the functional inactivation of TCAB1 and ALT status across all 38 samples analyzed by either IHC or WGS. D) Theoretical framework for the evolution of telomerase and ALT positive tumors during osteosarcomagenesis.

Article Snippet: For p53, slides were steamed in pH6.0 citrate-based unmasking solution (Cat# H-3300, Vector Laboratories) for 30 min, blocked with ReadyProbes Endogenous HRP and AP Blocking Solution (Invitrogen, #R37629) and Protein Block (#ab64226, abcam), then were incubated overnight at 4C in mouse monoclonal primary antibody against p53 (1:50 dilution, Cat# sc-126, Santa Cruz Biotechnology).

Techniques: Functional Assay